Cameras

WinFluor V4.0.3

Getting Started > Cameras > Cameras

 

The type of camera in use and camera settings are configured on the Camera page.

 

Camera

Camera: Selects the required camera support library for the camera attached to the system. Note. Before selecting a camera, ensure that the camera software drivers and support library are installed and the camera is connected and switched on.

Readout Speed: Select the camera CCD readout rate of multiple rates are supported (usually the highest)

COM Port: If the camera is controlled via a serial communications line, selects the computer COM port.

Tempr. Set Point: The Tempr. Set Point setting determines the target temperature for camera equipped with Peltier cooled CCDs.

Disable Exposure Time Checking: (Q Imaging cameras only) Tick this option to disable the checking of the camera exposure interval against the CCD readout time, allowing exposure intervals shorter than the reported readout time.

Exposure Trigger Output

Exposure Trigger Output: Selects the analog or digital output line to be used to trigger camera exposures or Internal camera triggering. The output line is connected to the external trigger input of the camera.

Active High / Low: Sets the polarity of the trigger pulse required by the camera (Active High – for a 0V-to-5V pulse or Active-Low – for a 5V-to-0V pulse.).

Trigger Delay: Determines the delay (ms) between the change of excitation wavelength (in multi-wavelength imaging) and the triggering of camera exposure.

Bulb Exposure Mode: When this mode is selected, camera exposure time is determined by the duration of the trigger pulse and can be varied from frame to frame. This option must be enabled to support the variable (% exposure) time in WinFluor's programmable excitation/emission wavelength control.

Clear CCD before exposure: Sets the camera to clear the CCD at the beginning of a exposure. (Note. Not all cameras support this option. Enabling this option may reduce maximum frame rate of the camera.)

Post-exposure CCD readout: Forces CCD readout before the next exposure can take place. (Note. This option reduces camera performance. This option is automatically selected for cameras which only support post exposure readout).

Extra Readout Time: In externally triggered exposure mode, camera exposure time is normally set to 90% of the inter-frame interval. The Extra Readout Time value (default 0) allocates extra time for CCD readout by reducing the exposure time of the camera.

 

Calibration

Lens magnification: Sets the magnification factor of the microscope objective lens.

Calibration bar: Sets the desired size (in microns) of the display calibration bar.

Calibration bar (width):  Sets the thickness of the displayed calibration bar (in pixels).

 

Split Image Labels

Defines the labels of the frames containing the upper and lower halves of the camera image when Split Image mode has been selected in the Record Images & Signals window.

Upper: Defines the frame label associated with upper section of the camera image.

Lower: Defines the frame label associated with lower section of the camera image.

 

Camera Dark Level

Sets the upper and lower limits of the camera dark level range used by the time lapse mode camera acquisition failure detector. When an acquired time lapse frame contains an image containing only pixels in the dark level acquisition is restarted. A dark level range of -1 to -1 disables this function.