Getting Started > Light Sources > Light Sources
The type of light source in use and its settings are configured on the Light Source page.
Light Source
Light Source: Specifies the type of computer-controllable light source attached to the system.
Wavelength Control Outputs (Range): Selects the group of analog or digital output lines used to control light source wavelength. Select the first output line in the group from the Start list and the last output line from the End list.
Shutter Control
Shutter closed wavelength: Sets the monochromator wavelength used when the excitation Off setting is selected in the WinFluor recording windows.This is typically chosen to be a wavelength blocked by the dichroic or barrier filters in the microscope epi-cube.
Opening time: Defines the time (ms) allowed for the shutter to open before each exposure (or sequence of exposures when multi-wavelength imaging) in the time lapse recording mode.
Frame blanking period: Defines the period of time (default 0 ms) for which illumination is turned off at the end of each camera acquisition frame.
Shutter Control Output: If the light source requires a shutter control line, select the analog or digital output line to be used to open/close the light source shutter from the Shutter Control list and set the polarity of the trigger pulse required by the shutter (Active High for a 0V-to-5V pulse or Active-Low for a 5V-to-0V pulse.).
Emission Filter
Control lines and settings for controlling a filter wheel placed in the emission light pathway, allowing the selection of specific emission wavebands to be selected along with as excitation light wavelength.
Filter Control Outputs: Selects the group of digital output lines used to control the emission filter. Select the first output line in the group from the Start list and the last output line from the End list. Output lines are connected to TTL filter selection inputs
Change Time: Specifies the time (ms) taken for the filter wheel to switch emission filters. Filter changes take place at the end of each exposure interval and the camera exposure is shortened by <change time> to avoid smearing of image.