Recording > Images + Signals > Z Axis Position
When a lens/stage positioner is available, the Z Axis Position group of settings defines the lens focal plane (Z) position.
The plane of focus can be shifted up our down using the Z Axis Position slider or moved to a specific position by entering the position (in microns) in the Z Axis Position box and pressing the return key.
Z Stack
The Z Stack option can be used to record a set of images at a series of focal planes.
To record a Z stack, enter the position of the initial image plane in the Start At box, the number of planes to be acquired in the No. Steps box and the distance between planes in the Step Size box. Tick the Enable option to enable the Z stack recording.