expression.SetSpreadsheetData(DataReference, SeriesByRows)
expression Required. An expression that returns a ChartSpace or ChChart object.
DataReference Required String. A reference to the range of cells to bind to. This can be in the form of a cell reference (A1:D5), or a defined name.
SeriesByRows Optional Boolean. Specifies whether or not each row represents a data series. Set this property to False if each column represents a data series.
Example
This example binds ChartSpace1 to cells A1:F25 in the first sheet of Spreadsheet1.
ChartSpace1.SetSpreadsheetData "Sheet1!A1:F25", False