Tecella Patch Clamp Amplifier Control Panel

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Getting Started > Amplifiers > Tecella Patch Clamp Amplifier Control Panel

 

The amplifier gain, compensation and current/voltage clamp mode of  Tecella patch clamp amplifiers can be set from this control panel.

 

Select

SetupTecella Patch Clamp

to display the Tecella Patch Clamp control panel.

 

Config: Selects the voltage/current clamp mode and stimulus current/voltage limits.

Input: Selects the amplifier input (None = No input, Head = headstage input, VModel  = 100 MOhm model cell, IModel = 1 MOhm model cell).

Gain: In voltage-clamp mode, selects the headstage feedback resistor. In current-clamp mode. selects amplifier gain.

Low pass filter: Slider selects cutoff frequency of Bessel low pass filter.

 

Compensation

Auto page: Automatic capacity/leak current compensation.

Click Auto Compensate to automatically compensate for pipette/cell capacity and cell leak conduction.

Click Junct. Pot. Auto Zero to compensate for the electrode junction potentials, setting the input current to zero.

Click Clear Compensation to set all compensation to zero.

Options

Tick the Use analog leak subtraction option to use the amplifier analog leak current subtraction circuits in the automatic leak current compensation.

Tick the Use digital leak current subtraction option to use the digital leak current in the automatic leak current compensation.

Tick the Use digital artefact removal option to use the digital artefact function in the automatic leak current compensation.

Tick Apply to all channels to apply automatical compensation to all amplifier channels (only applies to multi-channel amplifiers).

Compensation coefficient: Sets the compensation coefficient factor (0=optimal compensation, >0=under-compensation, <0 = over-compensation).

 

Capacity page: Capacity compensation settings in use. Can be adjusted by the user or set automatically using Auto Compensate.

 

Resistance page: Cell leak and pipette series resistance compensation settings in use. Can be adjusted by the user or set automatically using Auto Compensate.

 

Junction Pot. page: Electrode junction potential compensation settings in use. an be adjusted by the user or set automatically using Junct. Pot. Auto Zero.

 

Zap Cell: Click Zap Cell to apply a voltage pulse of amplitude set by Amplitude and duration set by Dur.