Signal Connections Table

Getting Started > Amplifiers > Signal Connections Table

The following table shows signal connections the required for the WinWCP-suppported amplifiers.

Amplifier outputs/input in panel (A) are connected to the laboratory interface input/outputs in the corresponding column in panel (B).
 

Note 1. When the Axopatch 200 is switched from voltage- to current-clamp mode, the Scaled Output signal to the primary channel (AI Ch.0 for Amplifier #1) changes from membrane current to voltage. To retain a current signal, the secondary channel (AI Ch.1 for Amplifier #1) of WinWCP must be switched manually from the Axopatch 200 10 Vm to the Axopatch 200 Im output (on the rear panel).

Note 2. Axon Multiclamp amplifiers support two separate amplifier channels (1 & 2). When using amplifier Ch.1, select Multiclamp 700A/B as Amplifier #1 and connect the Ch.1 Scaled and Raw/Secondary outputs to AI Ch.0 and AI Ch.1, respectively, and AO Ch.0 to Ch.1 EXT COMMAND. When using both channels, select Multiclamp 700A/B also as Amplifier #2 and connect the Ch.2 Scaled and Raw/Secondary outputs to AI Ch.2 and AI Ch.3 and AO Ch.1 to Ch.2 EXT COMMAND. (Axon Multiclamp amplifiers can only be selected as WinWCP Amplifiers #1 and #2.)

The Axon Multiclamp Commander software must be started up and running before WinWCP is started.

Note 3. The Heka EPC-8 gain and mode telegraphs are TTL digital signals provided via a 50 way IDC ribbon cable on the rear of the EPC-8. The signals in the table below must be connected to the digital inputs of the laboratory interface.

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