Signal Connections Table

WinWCP V5.3.8

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Getting Started > Amplifiers > Signal Connections Table

The following table shows signal connections the required for the WinWCP-suppported amplifiers.

 

Amplifier outputs/input in panel (A) are connected to the laboratory interface input/outputs in the corresponding column in panel (B).
 

A)

Amplifier Inputs/Outputs

Type of
Amplifier

Primary
Channel

Secondary
Channel

MODE

GAIN

Vcom

Manual gain
entry

Im Out

Vm Out

-

-

Vcommand In

Axopatch
1D

Scaled Output

10 Vm

-

Gain
Telegraph

Ext. Command

Axopatch
200

Scaled Output

10 Vm (VC mode)
Im (CC mode)

See Note.1

Mode
Telegraph

Gain
Telegraph

Ext.Command (front switched)

Multiclamp
700A

Scaled
Output

Raw
Output

-

- See Note 2

Ext.Command

Multiclamp
700B

Primary
Output

Secondary
Output

-

- See Note 2

Ext. Command

RK400

Iout

10 x Vm

-

-

Voltage
Command
Input

VP500

-

-

-

-

-

Heka EPC-7

Current
Monitor

Vcomm
Monitor

-

-

Stim Input
X10

Heka EPC-8

Current
Monitor

Vcomm
Monitor

-

See Note 3.

Stim Input
X10

Heka EPC-800

Current
Monitor

Vcomm
Monitor

Mode
Telegraph

Gain
Telegraph

Stim Input
X10

Cairn
Optopatch

Gain Out

Command
X10 Out

Pin 9
Pin 2 Gnd
37 way D socket

Gain
Telegraph Out

Command /10 In

Warner
PC501A

Im

Vm x10

-

Gain
Telegraph

CMD In

Warner
PC505B

Im

Vm x10

-

Gain
Telegraph

Command
In

Warner
OC725

I Monitor

Vm x10

-

Gain
Telegraph

Command
In ÷10

NPI SEC05LX

Current
Output

Potential
Output

-

Curr.
Sensitivity
Monitor

VC Command
Input /10

AM Systems
2400

Output

X10 Vm

Mode
Telegraph

Gain
Telegraph

External

÷50

Tecella

-

-

-

-

-

 

 

 

 

 

 

B)

Default Laboratory Interface Inputs/Outputs

Amplifer #1

AI Ch.0

AI Ch.1

AI Ch.6

AI Ch.7

AO Ch.0

Amplifer #2

AI Ch.2

AI Ch.3

AI Ch.14

AI Ch.15

AO Ch.1

Amplifer #3

AI Ch.4

AI Ch.5

AI Ch.13

AI Ch.12

AO Ch.2

Amplifer #4

AI Ch.6

AI Ch.7

AI Ch.10

AI Ch.11

AO Ch.3

 

Note 1. When the Axopatch 200 is switched from voltage- to current-clamp mode, the Scaled Output signal to the primary channel (AI Ch.0 for Amplifier #1) changes from membrane current to voltage. To retain a current signal, the secondary channel (AI Ch.1 for Amplifier #1) of WinWCP must be switched manually from the Axopatch 200 10 Vm to the Axopatch 200 Im output (on the rear panel).

 

Note 2. Axon Multiclamp amplifiers support two separate amplifier channels (1 & 2). When using amplifier Ch.1, select Multiclamp 700A/B as Amplifier #1 and connect the Ch.1 Scaled and Raw/Secondary outputs to AI Ch.0 and AI Ch.1, respectively, and AO Ch.0 to Ch.1 EXT COMMAND. When using both channels, select Multiclamp 700A/B also as Amplifier #2 and connect the Ch.2 Scaled and Raw/Secondary outputs to AI Ch.2 and AI Ch.3 and AO Ch.1 to Ch.2 EXT COMMAND. (Axon Multiclamp amplifiers can only be selected as WinWCP Amplifiers #1 and #2.)

The Axon Multiclamp Commander software must be started up and running before WinWCP is started.

Note 3. The Heka EPC-8 gain and mode telegraphs are TTL digital signals provided via a 50 way IDC ribbon cable on the rear of the EPC-8. The signals in the table below must be connected to the digital inputs of the laboratory interface.

 

Heka EPC-8 Digital gain telegraph connections

 

Gain 0

Gain 1

Gain 2

Range 0

Range 1

Gnd

EPC-8 50 way IDC socket

5

7

9

1

17

19

Digital Input Channel

0

1

2

3

4

Gnd

 

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