Patch Clamp Signal Connections

WinFluor

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Getting Started > Patch Clamp Signal Connections

 

To configure WinFluor to acquire cell current and voltage signals from a patch clamp, signal connections must be made between the patch clamp amplifier outputs (current, voltage, gain telegraph and mode telegraph) and inputs (command voltage) and appropriate analogue inputs and outputs on the PCI-6229 analogue interface board.

The following signals connections are required:

 

Patch Clamp Amplifier

 

Analogue Interface Inputs/Outputs

1)

Current Out

 

AI0

2)

Membrane Potential Out

 

AI1

3)

Gain Telegraph Out

 

AI7

4)

Mode Telegraph Out

 

AI6

5)

Command Voltage In

 

Vout 0

Current and voltage outputs (1,2) must be connected to interface inputs AI0 and AI1, respectively. Telegraph connections (3, 4) are not be required with patch clamps which do not provide gain and/or mode telegraphs, or communicate gain information via USB or other communications lines.

The command voltage input is connected to voltage stimulus channel Vout 0 (which is usually configured as interface digital/analogue output AO0). Table 1 lists the required connections and signal output names for the patch and voltage clamps currently supported by WinFluor.

Table 1 Patch clamp signal connections

 

PatchClamp

Signals

Telegraphs

Command
Voltage O/P

AI0

AI1

AI6

AI7

Voltage #1

Axopatch
1D

Scaled Output

10 Vm

-

Gain
Telegraph

Ext.
Command

Axopatch
200

Scaled Output

10 Vm (VC mode)
Im (CC mode)

See Note.1

Mode
Telegraph

Gain
Telegraph

Ext.
Command

Multiclamp
700A

Scaled
Output

Raw
Output

-

- See Note 2

Ext.
Command

Multiclamp
700B

Primary
Output

Secondary
Output

-

- See Note 2

Ext.
Command

RK400

Iout

10 x Vm

-

-

Voltage
Command
Input

VP500

-

-

-

-

-

Cairn
Optopatch

Gain Out

Command
X10 Out

Pin 11 (37 way D socket) to Dig. In 0

Gain
Telegraph Out

Command /10 In

Warner
PC501A

Im

Vm x10

-

Gain
Telegraph

CMD In

Warner
PC505B

Im

Vm x10

-

Gain
Telegraph

Command
In

Warner
OC725

I Monitor

Vm x10

-

Gain
Telegraph

Command
In ÷10

NPI SEC05LX

Current
Output

Potential
Output

-

Curr.
Sensitivity
Monitor

VC Command
Input /10

AM Systems
2400

Output

X10 Vm

-

Gain
Telegraph

External

÷50

 

Note 1. When the Axopatch 200 is switched from voltage- to current-clamp mode, the Scaled Output signal to ADC1 is changed automatically from membrane current to voltage. To retain a current signal, ADC1 must be switched manually from the 10 Vm output of the Axopatch 200 to the Im. Output.

Note 2. WinFluor obtains channel gain information from the Axon Multiclamp commander software. Multiclamp Commander must be started up and running before WinFluor is started.

 

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